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                    Amino Acids and Peptides

                    簡要描述:The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSY

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                    詳細介紹

                    Amino Acid and Peptide Analysis

                     

                    UltraSep ESD AP

                      

                    The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSYLchloride shows advantages.

                    Comparison of different procedures for derivatization

                    Procedure

                    Detection

                    Limit of detection

                    Remarks

                    DABS

                    Absorption
                    436 nm

                    < 1 pmol

                    Stable derivatives; primary and secondary amino acids can be determined

                    OPA

                    Fluorescence
                    330/440 nm

                    < 1 pmol

                    Pro, Hypro and Cys cannot be determined 

                    FMOC

                    Fluorescence
                    265/315 nm

                    < 1 pmol

                    His and Cys will be detected only in part

                    DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
                    OPA = o-Phthalaldehyde
                    FMOC = 9-Fluorenylmethoxycarbonylchloride

                     

                    Examples of Analyzed Compounds

                    Analyzed in Application

                    Go to

                     

                    Alanine (Ala)

                    AA

                     

                     

                    Arginine (Arg)

                    AA

                    AA

                     

                    Aspartic acid (Asp)

                    AA

                     

                     

                    Cystine (Cys2)

                    AA

                    EPHARM6

                     

                    Glutamic acid (Glu)

                    AA

                     

                     

                    Glycine (Gly)

                    AA

                     

                     

                    Glutathione

                    EPHARM6

                     
                     

                    Histidine (His)

                    AA

                     

                     

                    Isoleucine (Ile)

                    AA

                     

                     

                    Leucine (Leu)

                    AA

                     

                     

                    Lysine (Lys)

                    AA

                     

                     

                    Methionine (Met)

                    AA

                     

                     

                    Ornithine (Orn)

                    AA

                     

                     

                    Phenylalanine (Phe)

                    AA

                     

                     

                    Proline (Pro)

                    AA

                     

                     

                    Serine (Ser)

                    AA

                     

                     

                    Threonine (Thr)

                    AA

                     

                     

                    Tyrosine (Tyr)

                    AA

                     

                     

                    Valine (Val)

                    AA

                     

                     

                    COLUMNS and  PRICES  ()
                     Column size (mm)
                    250 x 4250 x 3250 x 2125 x 4125 x 3125 x 2
                    UltraSep ESD AP360.00340.00355.00320.00295.00290.00
                     

                    Size (mm)

                     
                    10 x 410 x 310 x 2
                    Precolumn cartridges
                    (Price per pack = 5 pieces)
                    170.00170.00170.00

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